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These metrics are regularly updated to reflect usage leading up to the last few days. Citations are the number of other articles citing this article, calculated by Crossref and updated daily.
Find more information about Crossref citation counts. The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric. Find more information on the Altmetric Attention Score and how the score is calculated.
Recent advances in Synthetic Biology have yielded standardized and automatable DNA assembly protocols that enable a broad range of biotechnological research and development. Unfortunately, the experimental design required for modern scar-less multipart DNA assembly methods is frequently laborious, time-consuming, and error-prone. The key innovations of the j5 design process include cost optimization, leveraging DNA synthesis when cost-effective to do so, the enforcement of design specification rules, hierarchical assembly strategies to mitigate likely assembly errors, and the instruction of manual or automated construction of scar-less combinatorial DNA libraries.
Using a GFP expression testbed, we demonstrate that j5 designs can be executed with the SLIC, Gibson, or CPEC assembly methods, used to build combinatorial libraries with the Golden Gate assembly method, and applied to the preparation of linear gene deletion cassettes for E. The DNA assembly design algorithms reported here are generally applicable to broad classes of DNA construction methodologies and could be implemented to supplement other DNA assembly design tools. Taken together, these innovations save researchers time and effort, reduce the frequency of user design errors and off-target assembly products, decrease research costs, and enable scar-less multipart and combinatorial DNA construction at scales unfeasible without computer-aided design.
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For permission to reproduce, republish and redistribute this material, requesters must process their own requests via the RightsLink permission system. View Author Information. Cite this: ACS Synth. Article Views Altmetric -. Citations Supporting Information. Cited By. This article is cited by publications. Samuel C. Curran, Jose H.
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ACS Synthetic Biology , 3 8 , ACS Synthetic Biology , 2 11 , Sleight and Herbert M. ACS Synthetic Biology , 2 9 , ACS Synthetic Biology , 2 5 , Stevens and Chris J. ACS Synthetic Biology , 1 8 , A novel gene expression system for Ralstonia eutropha based on the T7 promoter. Incha, Mitchell G. Thompson, Jacquelyn M. Pearson, Matthias Schmidt, Jennifer W. Gin, Christopher J. Petzold, Adam M. Deutschbauer, Jay D. Leveraging host metabolism for bisdemethoxycurcumin production in Pseudomonas putida.
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j5 DNA assembly design automation software.
j5 DNA Assembly Design Automation Software
Skip to search form Skip to main content You are currently offline. Some features of the site may not work correctly. DOI: Hillson and Rafael D. Rosengarten and Jay D. Hillson , Rafael D.