It is known that targeting of antigen to antigen presenting cells APC increases immune responses. However, it is unclear if more than one APC-specific targeting unit in the antigenic molecule will increase responses. To address this issue, we have here made heterodimeric vaccine molecules that each express four different fusion subunits. The bacterial ribonuclease barnase and its inhibitor barstar interact with high affinity, and the barnase-barstar complex was therefore used as a dimerization unit. C-terminal antigenic fusions were either the fluorescent protein mCherry or scFv derived from myeloma protein M The heterodimeric vaccine molecules were formed both in vitro and in vivo.

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Are you sure you want to Yes No. Browse by Genre Available eBooks No Downloads. Views Total views. Actions Shares. Embeds 0 No embeds. No notes for slide. Barnase barstar system 1. Markers 4. Flanking marker Gene of interest 6. Introduction or Marker assisted selection 9. Prerequisite for using MAS General Steps in MAS Variety of approaches MAS Marker-assisted backcrossing MAB Pyramiding Fact About molecular breeding……. Features Achievements Tapetum Tissue Bargene Mariani et al.

Induced GMS Barnase gene is cytotoxic killing the tapetul cells, thus preventing pollen development result transgenic male sterility. Each grandparent has each part of Barnase. Two-Component System Fertility restoration Barstar Mariani et al.

Most Attractive than Barnase Barstar system Banga et. Gupta P. Plant Breeding Principles and Methods, 5. Marker-assisted selection:an approach for precision plant breeding in the twenty-first century-Bertrand C.

Y Collard and David J Mackill, 6. C,Hybrid Seed Production, 7. You just clipped your first slide! Clipping is a handy way to collect important slides you want to go back to later. Now customize the name of a clipboard to store your clips. Visibility Others can see my Clipboard. Cancel Save.


Engineered Male Sterility by Early Anther Ablation Using the Pea Anther-Specific Promoter PsEND1

Barnase a portmanteau of "BActerial" "RiboNucleASE" is a bacterial protein that consists of amino acids and has ribonuclease activity. It is synthesized and secreted by the bacterium Bacillus amyloliquefaciens , but is lethal to the cell when expressed without its inhibitor barstar. The inhibitor binds to and occludes the ribonuclease active site, preventing barnase from damaging the cell's RNA after it has been synthesized but before it has been secreted. Barnase has no disulfide bonds , nor does it require divalent cations or non-peptide components to fold. This simplicity, in combination with its reversible folding transition, means that barnase has been extensively studied in order to understand how proteins fold.


Barnase and Barstar: Two Small Proteins to Fold and Fit Together

Barnase and barstar are the extracellular ribonuclease and its intracellular inhibitor produced by Bacillus amyloliquefaciens. Both are small single-chain proteins and thus are suitable for application to the study of how a protein's sequence directs its fold. Barnase has neither disulfide bonds nor non-peptide components and unfolds reversibly in what closely approximates a two-state reaction. The genes for both these proteins have been cloned in E. Expression of barstar is necessary to counter the lethal effect of expressed active barnase. Site-directed mutagenesis is being used to answer specific and general questions relating to protein folding and protein-protein interaction. This site needs JavaScript to work properly.


Genetic engineered male sterility has different applications, ranging from hybrid seed production to bioconfinement of transgenes in genetic modified crops. The impact of this technology is currently patent in a wide range of crops, including legumes, which has helped to deal with the challenges of global food security. Production of engineered male sterile plants by expression of a ribonuclease gene under the control of an anther- or pollen-specific promoter has proven to be an efficient way to generate pollen-free elite cultivars. In the last years, we have been studying the genetic control of flower development in legumes and several genes that are specifically expressed in a determinate floral organ were identified. This expression pattern has been assessed in both model plants and crops tomato, tobacco, oilseed rape, rice, wheat using genetic constructs carrying the PsEND1 promoter fused to the uidA reporter gene.



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