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For in vivo studies, a novel model for antibody-induced thrombocytopenia and thrombosis was established. Anti-GPIX antibodies dose-dependently caused thrombocytopenia and pulmonary thrombosis in hFcR-transgenic but not wild-type mice.

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Optional text in email:. Save Cancel. Create a file for external citation management software Create file Cancel. Full-text links Cite Favorites. A Representative histograms for platelets from a WT…. A Representative histograms for platelets from a WT mouse dashed black trace and a mouse transgenic for the hFcR black trace stained with IV.

Only mice with a platelet mean fluorescence signal IV. A-B Representative aggregation traces in response…. A-B Representative aggregation traces in response to anti-CD9. Traces are representative of 3 independent experiments. C Dose-response of anti—CD9-induced platelet aggregation. A Time…. Bottom panel Total Rap1 as loading control. Traces are representative of 4 independent experiments.

A Aggregation…. Aggregation responses of hFcR platelets to either 0. Representative of 3 independent experiments. Figure 5 5 Antiplatelet antibody-induced thrombocytopenia and thrombosis….

Figure 5 13 Antiplatelet antibody-induced thrombocytopenia and thrombosis in vivo. A Platelet count of hFcR transgenic….

Antiplatelet antibody-induced thrombocytopenia and thrombosis in vivo. A Platelet count of hFcR transgenic mice solid line injected with 0. Results shown are percentage platelet count compared with baseline.

Arrows point at fibrin and platelet-rich thrombi in the lung vasculature. Images are representative of 3 independent experiments. A-B Platelet counts in whole…. Results shown are mean fluorescence intensity a. See this image and copyright information in PMC. Amirkhosravi A, et al.

J Thromb Haemost. Epub Oct Stolla M, et al. Cifuni SM, et al. Epub Jun Stefanini L, Bergmeier W. Stefanini L, et al. J Mol Med Berl. Epub Oct 1. Show more similar articles See all similar articles.

Takino JI, et al. Sci Rep. Heparin-induced thrombocytopenia HIT : Identification and treatment pathways. Fathi M. Glob Cardiol Sci Pract. Small molecule targeting the Rac1-NOX2 interaction prevents collagen-related peptide and thrombin-induced reactive oxygen species generation and platelet activation.

Akbar H, et al. Epub Aug Epub Jun 4. Platelets at the Vascular Interface. Bergmeier W, Stefanini L. Bergmeier W, et al. Version 2. Res Pract Thromb Haemost. Epub Dec Show more "Cited by" articles See all "Cited by" articles. Publication types Research Support, N. Research Support, Non-U. Gov't Actions. MeSH terms Animals Actions. Blotting, Western Actions. Cell Separation Actions. Disease Models, Animal Actions.

Flow Cytometry Actions. Humans Actions. Mice Actions. Mice, Knockout Actions. Mice, Transgenic Actions. Guanine Nucleotide Exchange Factors Actions. P2ry12 protein, mouse Actions. Rasgrp2 protein, mouse Actions. Receptors, IgG Actions. Receptors, Purinergic P2Y12 Actions. Copy Download.


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